Table 5: Effect of the temperature on the apparent protein concentration.
T ( °C) |
nD |
Net nD a) |
Protein (mg/mL) b) |
|
Plasma |
10 kDa permeat |
|||
17.5 |
1.34617 |
1.33506 |
0.01111 |
57.3 |
20.0 |
1.34592 |
1.33484 |
0.01108 |
57.1 |
22.5 |
1.34566 |
1.33459 |
0.01107 |
57.1 |
25.0 |
1.34538 |
1.33432 |
0.01106 |
57.0 |
27.5 |
1.34507 |
1.33403 |
0.01104 |
56.9 |
30.0 |
1.34476 |
1.33372 |
0.01104 |
56.9 |
Remarks: a) Net nD is calculated as the difference between the RIs determined for plasma and its corresponding 10 kDa permeate; b) A dn/dc value of 0.0001942 was used for the calculation of the protein concentration.