Table 7: Determination of the confidence intervals for quality controls.

Quality Control

Equation for the

confidence interval

Mean

t (0.01, DF = 5)

SD

Confidence interval (99%)

NC

mean (A405nm) + t (0.01, 5df) × SD

0.063

3.365

0.0104

A405nm ≤ 0.098

LQC

mean (LQC/NC) ± t (0.01, 5df) × SD

3.980

4.032

0.4214

2.28 ≤ LQC/NC ≤ 5.68

HQC

mean (HQC/NC) ± t (0.01, 5df) × SD

13.80

4.032

1.6531

7.13 ≤ HQC/NC ≤ 20.5

NC: Negative control; LQC: Low positive quality control; HQC: High positive quality control.