Table 1: Results for the various coating conditions.
|
Attribute |
Coating buffer pH 9.5 (µg/mL) |
Coating buffer pH 7.2 (µg/mL) |
||||||
|
1 |
5 |
10 |
20 |
1 |
5 |
10 |
20 |
|
|
OD of D1 (4.6 mU FXI/mL) |
1.377 |
1.367 |
1.437 |
1.507 |
1.220 |
1.230 |
1.259 |
1.305 |
|
Blank to D1 ratio (%) |
0.80 |
0.80 |
0.97 |
1.00 |
0.98 |
0.89 |
0.95 |
1.69 |
|
Slope |
0.8531 |
0.8642 |
0.8698 |
0.8770 |
0.8739 |
0.8688 |
0.8720 |
0.8894 |
|
Correlation coefficient r |
0.9984 |
0.9996 |
0.9996 |
0.9995 |
0.9983 |
0.9995 |
0.9993 |
0.9989 |
|
Relative total error (RTE) |
15.0 |
7.4 |
7.2 |
8.4 |
15.2 |
8.3 |
9.4 |
12.1 |
|
OD/mU FXI |
0.409 |
0.382 |
0.394 |
0.409 |
0.353 |
0.344 |
0.354 |
0.356 |
Remarks: Coating buffer with pH values of 9.5 and 7.2 was used to coat anti-FXI IgG concentrations of 1 to 20 μg/mL. OD gives the blank-corrected ODs. The dose-response curves, obtained for the human reference plasma preparation, combined FXI concentrations ranging from 0.14 (D6) to 4.6 (D1) mU FXI/mL. The attribute OD/mU FXI was calculated by insertion into the equation of the log-log regression curve.