Table 2: Results parameters of the calibration curve for linearity.

Nominal Concentration

Batch No: 1

Batch No: 2

Batch No: 3

Mean Conc. Found

ng/mL)

CV (%)

RE (%)

(ng/mL)

Conc. Calc.

(ng/mL)

RE (%)  

Conc. Calc.

(ng/mL)

RE

(%)

Conc. Calc.

(ng/mL)

RE

(%)

80

81.003

1.254

81.652

2.065

80.933

1.166

81.196

0.489

1.495

160

157.254

-1.716

155.385

-2.884

158.291

-1.068

156.977

0.938

-1.890

1000

950.323

-4.968

923.096

-7.690

923.346

-7.665

932.255

1.678

-6.774

5000

4967.102

-0.658

4866.849

-2.663

4882.811

-2.344

4905.587

1.098

-1.888

10000

9858.516

-1.415

9983.440

-0.166

9768.969

-2.310

9870.308

1.091

-1.297

20000

20484.544

2.423

20757.970

3.790

20621.681

3.108

20621.398

0.663

3.107

27000

27414.217

1.534

27639.417

2.368

27603.290

2.234

27552.308

0.439

2.046

30000

31063.874

3.546

31554.001

5.180

32063.658

6.879

31560.511

1.584

5.202

Conc: Concentration; CV: Coefficient of Variation; RE: Relative error (Accuracy).