Table 1: Historical RI increment values for human plasma/serum protein.
Sample |
Remarks (solvent, conditions, calibration) |
dn/dc [mL/mg] |
Reference |
Plasma |
19 plasma pools for fractionation |
0.0001833 |
[2] |
Serum |
0.9% NaCl |
0.0001784 |
[3] |
Serum |
15 normal sera, UF permeate-subtracted, Kjeldahl |
0.000194 |
[4] |
Serum |
69 sera, gravimetric |
0.000196 |
[5] |
Serum |
N/D |
0.000195 |
[6] |
Serum |
20 pathological sera |
0.000210 |
[7] |
Serum |
32 pathological sera |
0.000197 |
[8] |
Serum |
10 pathological sera |
0.000200 |
[9] |
Serum |
47 pathological sera |
0.000201 |
[9] |
Serum |
39 sera, gravimetric |
0.0001906 |
[10] |
Serum |
4 h after venipuncture (VP) |
0.000189 |
[11] |
Serum |
Veronal buffer (VP), pH = 8.6 (dialyzed, 4 h after VP) |
0.000193 |
[12] |
Serum |
VP, pH = 8.6 (dialyzed, 24 h at room temperature) |
0.000204 |
[12] |
Serum |
Personal communication to authors |
0.000179 |
[13] |
Serum |
Recalculated using a protein to N ratio of 6.54 |
0.000187 |
[14] |
Remarks: Robertson (1912) determined a dn/dc value of 0.000195 mL/mg for bovine serum protein [15].