Table 4: Dilution linearity of plasma protein.
Sample/Dilution |
J-357 digital |
TS-D digital |
Goldberg TS |
||
mg/mL a) |
mg/mL a) |
mg/mL SP (direct) b) |
mg/mL SP (20 °C) c) |
mg/mL SP d) |
|
Permeate |
0.0 |
0 |
7 |
9 (23.7 °C) |
ND |
0.10 |
16.8 |
17 |
21 |
22 (22.6 °C) |
~ 20 |
0.15 |
25.2 |
26 |
26 |
28 (23.0 °C) |
26 |
0.20 |
34.0 |
34 |
35 |
37 (23.3 °C) |
35 |
0.30 |
49.6 |
49 |
51 |
53 (23.4 °C) |
51 |
0.40 |
66.5 |
66 |
67 |
70 (23.8 °C) |
68 |
0.50 |
82.9 |
82 |
84 |
86 (23.8 °C) |
85 |
0.60 |
99.3 |
98 |
100 |
102 (23.9 °C) |
102 |
0.70 |
115.9 |
115 |
117 |
119 (24.1 °C) |
118 |
0.80 |
132.4 |
131 |
133 |
136 (24.1 °C) |
136 |
0.90 |
150.4 |
150 |
152 |
155 (24.2 °C) |
~ 154 |
Concentrate |
168.3 |
169 |
171 |
173 (24.3) |
ND |
Remarks: ND stands for not determined, SP for serum protein: a) Calculated protein concentrations using the dn/dc value of 0.000192 for the permeate-corrected RI values; b) Serum protein concentrations as calculated by the instrument without temperature and permeate correction; c) Serum protein concentrations as obtained after temperature compensation automatically done by the instrument. Numbers in brackets show the temperature actually measured; d) Serum protein concentrations as obtained by the Goldberg TS device.