Table 5: Effect of the temperature on the apparent protein concentration.

T ( °C)

nD

Net nD a)

Protein (mg/mL) b)

Plasma

10 kDa permeat

17.5

1.34617

1.33506

0.01111

57.3

20.0

1.34592

1.33484

0.01108

57.1

22.5

1.34566

1.33459

0.01107

57.1

25.0

1.34538

1.33432

0.01106

57.0

27.5

1.34507

1.33403

0.01104

56.9

30.0

1.34476

1.33372

0.01104

56.9

Remarks: a) Net nD is calculated as the difference between the RIs determined for plasma and its corresponding 10 kDa permeate; b) A dn/dc value of 0.0001942 was used for the calculation of the protein concentration.