Table 2: Regression equations for FVS at fixed time and 25 °C.

Time (min)

Regression equation

Correlation coefficient (r)

Linear range (μg/mL)

5

A = 0.0165C + 0.0337

0.9989

3.0-36.0

10

A = 0.0196C + 0.0439

0.9996

3.0-36.0

15

A = 0.0211C + 0.0477

0.9998

3.0-36.0

20

A = 0.0215C + 0.0566

0.9996

3.0-36.0

25

A = 0.0220C + 0.0595

0.9996

3.0-36.0

30

A = 0.0225C + 0.0584

0.9997

3.0-36.0

A: Absorbance; C: Concentration.